It also eliminates sample-to-sample variations and edge effects.
This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure ').
When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Unique rotary design for outstanding performance The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure '). The Type-it HRM PCR Kit reliably and accurately detects gene mutations and SNPs. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA. Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure ' for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure ' for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching. The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM. The average C T value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%.
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